AMBELBase-r1 Transcrtiptome

AMBELBase-r1 ( aka AMBEL-PO8A3t-20161018-FDP ) reference transcriptome dataset51242 sequences- Protocol:1. PacBIO RS_IsoSeq.1 assembly per IsoSeq Sized SMRTCells2. Quiver Polished high QV Consensus Isoforms strict redundancy removal3. Clustering (nrcl mini 99, maxo 50nt)4. Frameshift and orientation correction using FrameDP pipeline (dataset UniprotPlants + Sunflower XRQ r1.2)5. Orientation check using RNASeq mapping : GFF3 tag (insert_fwd, insert_rev, orientation)- BUSCO 3.0.2 analysis (dataset embryophyta_odb9)INFO C:80.2% (S:34.2%,D:46.0%),F:2.6%,M:17.2%,n:1440INFO 1155 Complete BUSCOs (C)INFO 493 Complete and single-copy BUSCOs (S)INFO 662 Complete and duplicated BUSCOs (D)INFO 38 Fragmented BUSCOs (F)INFO 247 Missing BUSCOs (M)INFO 1440 Total BUSCO groups searched