Identification of microRNA targets from Bletilla striata--degradome-seq

In this study, we performed high-throughput degradome sequencing (degradome-seq) to identify the microRNA (miRNA) targets from three organs (root, stem and leaf) of Bletilla striata. Firstly, the de novo assembled transcripts were subject to target prediction based on the lists of miRNAs discovered from Bletilla striata. Then, the degradome-seq data was used to validate the predicted miRNA--target pairs by using target plots (t-plots).