Microsatellite genetic characterization of Silene ciliata Poiret (Caryophyllaceae)
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We collected S. ciliata leaf tissue from 20 genets per population for genetic analysis (n = 180).
DNeasy Plant minikit (QIAGEN, Valencia, USA) was used for DNA extraction of 10–20 mg of dried S. ciliata tissue.
Based on a previous study (García-Fernández et al. 2012) we selected eight microsatellite loci for genotyping: Sci1224, Sci1208, Sci0106, Sci1443, EST-2HTS, EST-37HTS, EST-G34D06 and EST-G47A02.
PCR protocols were performed as described in García-Fernández et al. (2012).
We genotyped all samples in an automated DNA sequencer (ABI PRISM 3730, Applied Biosystems, California, USA) in Parque Científico de Madrid (Madrid, Spain).
GeneMarker version 1.85 (SoftGenetics, StateCollege, Pennsylvania, USA) was used for fragment size determination.
By re-amplifying and re-scoring 20% of the samples we evaluated genotyping accuracy
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e-cienciaDatos
创建时间：
2018-06-15
