Table_1_Single-Cell RNA-seq Reveals Characteristics of Malignant Cells and Immune Microenvironment in Subcutaneous Panniculitis-Like T-Cell Lymphoma.xlsx

BackgroundSubcutaneous panniculitis-like T-cell lymphoma (SPTCL) is a malignant primary T-cell lymphoma that is challenging to distinguish from autoimmune disorders and reactive panniculitides. Delay in diagnosis and a high misdiagnosis rate affect the prognosis and survival of patients. The difficulty of diagnosis is mainly due to an incomplete understanding of disease pathogenesis.MethodsWe performed single-cell RNA sequencing of matched subcutaneous lesion tissue, peripheral blood, and bone marrow from a patient with SPTCL, as well as peripheral blood, bone marrow, lymph node, and lung tissue samples from healthy donors as normal controls. We conducted cell clustering, gene expression program identification, gene differential expression analysis, and cell-cell interaction analysis to investigate the ecosystem of SPTCL.ResultsBased on gene expression profiles in a single-cell resolution, we identified and characterized the malignant cells and immune subsets from a patient with SPTCL. Our analysis showed that SPTCL malignant cells expressed a distinct gene signature, including chemokines families, cytotoxic proteins, T cell immune checkpoint molecules, and the immunoglobulin family. By comparing with normal T cells, we identified potential novel markers for SPTCL (e.g., CYTOR, CXCL13, VCAM1, and TIMD4) specifically differentially expressed in the malignant cells. We also found that macrophages and fibroblasts dominated the cell-cell communication landscape with the SPTCL malignant cells.ConclusionsThis work offers insight into the heterogeneity of subcutaneous panniculitis-like T-cell lymphoma, providing a better understanding of the transcription characteristics and immune microenvironment of this rare tumor.

背景：皮下脂膜炎样T细胞淋巴瘤（SPTCL）是一种恶性原发性T细胞淋巴瘤，其诊断过程中难以与自身免疫性疾病和反应性脂膜炎相区分。诊断的延迟以及高误诊率显著影响了患者的预后和生存率。诊断的困难主要源于对疾病发病机制的认知不完整。方法：我们对一名SPTCL患者的匹配皮下病变组织、外周血和骨髓进行了单细胞RNA测序，以及对健康捐献者的外周血、骨髓、淋巴结和肺组织样本进行了正常对照。我们进行了细胞聚类、基因表达程序识别、基因差异表达分析和细胞间相互作用分析，以探究SPTCL的生态系统。结果：基于单细胞分辨率的基因表达谱，我们识别并描述了SPTCL患者中的恶性细胞和免疫亚群。我们的分析显示，SPTCL恶性细胞表达独特的基因特征，包括趋化因子家族、细胞毒性蛋白、T细胞免疫检查点分子和免疫球蛋白家族。通过与正常T细胞的比较，我们确定了SPTCL中特异性差异表达的潜在新型标记物（例如，CYTOR、CXCL13、VCAM1和TIMD4）。我们还发现，巨噬细胞和成纤维细胞在SPTCL恶性细胞之间的细胞间通讯景观中占主导地位。结论：本研究揭示了皮下脂膜炎样T细胞淋巴瘤的异质性，为理解这种罕见肿瘤的转录特征和免疫微环境提供了新的见解。