Protocol for Quantifying Silent Ribosome Induction using Polysome Profiling
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https://www.ncbi.nlm.nih.gov/sra/SRP648844
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资源简介：
Translationally silent ribosomes have become an important focus in RNA biology, yet their quantification remains challenging. Lacking mRNAs, they represent a hibernating ribosome state. This protocol outlines how to measure silent ribosome induction under diverse conditions by analyzing RNA from polysome-profiling fractions in yeast and mammalian cells, using RNA-seq with removal of anomalously amplified rRNAs and qPCR for validation. Overall design: For an example of the execution of this protocol, please refer to Rahaman, et. al.1
创建时间：
2025-11-28
