Gene expression profiling of shCtrl and shCNN2 RKO cells from Clariom S array.

CNN2 is identified as a tumor promotor in the development of colorectal cancer. Herein, gene expression in RKO cells infected with shCtrl and shCNN2 (for CNN2 knockdown) was detected with a Clariom S array. Gene expression profiling of shCtrl and shCNN2 RKO cells was acquired and analyzed. Differentially expressed genes were identified based on fold change of mean of expression (fold change ≥ 1.3) and FDR (< 0.05) from P value calculated based on linear model of empirical Bayesian distribution. In total, 523 upregulated genes and 648 downregulated genes were characterized. Gene expression in shCNN2 (CNN2 knockdown) RKO cells and shCtrl (negative control) RKO cells was detected using Human gene expression array. Firstly, total RNA was extracted and RNA quality and integrity was determined by Nanodrop 2000 (Thremo Fisher Scientific) and Agilent 2100 and Agilent RNA 6000 Nano Kit (Agilent). Then, gene chip was performed with Clariom S array According to the instructions.