Data from: Primers for Castilleja and their utility across Orobanchaceae: II. Single-copy nuclear loci

Premise of the study: We developed primers targeting nuclear loci in Castilleja with the goal of reconstructing the evolutionary history of this challenging clade. These primers were tested across other major clades in Orobanchaceae to assess their broader utility. Methods and Results: We assembled low-coverage genomes for three taxa in Castilleja and developed primer combinations for the single-copy conserved ortholog set (COSII) and the pentatricopeptide repeat (PPR) gene family. These primer combinations were designed to take advantage of the Fluidigm microfluidic PCR platform and are well suited for high-throughput sequencing applications. Eighty-seven primers were designed for Castilleja, and 27 were found to have broader utility in Orobanchaceae. Conclusions: These results demonstrate the utility of these primers, not only across Castilleja, but for other lineages within Orobanchaceae as well. This expanded molecular toolkit will be an asset to future phylogenetic studies in Castilleja and throughout Orobanchaceae.研究背景：我们开发了靶向火焰草属（Castilleja）核基因座的引物，旨在解析这一疑难演化支的演化历史。同时，我们将这些引物在列当科（Orobanchaceae）的其他主要演化支中开展测试，以评估其更广范围的应用潜力。 方法与结果：我们组装了火焰草属3个类群的低覆盖度基因组，针对单拷贝保守同源基因集（COSII）以及五肽重复（PPR）基因家族开发了引物组合。该类引物组合针对Fluidigm微流控PCR平台设计，十分适配高通量测序应用。我们共为火焰草属设计了87对引物，其中27对在列当科中展现出更广泛的应用价值。 结论：本研究结果证实，这些引物不仅可在火焰草属内通用，还可应用于列当科内的其他类群。这套扩展的分子工具包将为未来火焰草属及列当科内的系统发育研究提供重要助力。