Dataset for: Characterization of Circulating Steroid Hormone Profiles in the Bottlenose Dolphin (Tursiops truncatus) by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

This dataset contains liquid chromatography tandem mass spectrometry (LC-MS/MS) data from common bottlenose dolphin plasma. These data were collected between January and December 2017 to examine the use of reverse phase solid phase extraction (SPE) coupled to liquid chromatography tandem mass spectrometry (LC-MS/MS) for the simultaneous, precise (< 15 % relative standard deviation), and accurate (between 70 % and 120% recovery of spiked quantities) measurement of steroid hormones in dolphin plasma. This dataset includes data from three individual experiments using three different sample sets. The first is a spike recovery experiment performed with free-ranging bottlenose dolphin plasma collected in September 2017, the second is an endogenous precision experiment using pooled samples collected in 2012 from dolphins maintained by the United States Navy, and the final experiment is a matrix (serum/plasma) comparison utilizing samples collected from free-ranging dolphins between 2014 and 2016. This dataset supports the publication: Galligan, T. M., Schwacke, L. H., Houser, D. S., Wells, R. S., Rowles, T., & Boggs, A. S. P. (2018). Characterization of circulating steroid hormone profiles in the bottlenose dolphin (Tursiops truncatus) by liquid chromatography–tandem mass spectrometry (LC–MS/MS). General and Comparative Endocrinology, 263: 80–91. doi:10.1016/j.ygcen.2018.04.003