Dataset for: Characterization of Circulating Steroid Hormone Profiles in the Bottlenose Dolphin (Tursiops truncatus) by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
收藏资源简介：
This dataset contains liquid chromatography tandem mass spectrometry (LC-MS/MS) data from common bottlenose dolphin plasma. These data were collected between January and December 2017 to examine the use of reverse phase solid phase extraction (SPE) coupled to liquid chromatography tandem mass spectrometry (LC-MS/MS) for the simultaneous, precise (< 15 % relative standard deviation), and accurate (between 70 % and 120% recovery of spiked quantities) measurement of steroid hormones in dolphin plasma. This dataset includes data from three individual experiments using three different sample sets. The first is a spike recovery experiment performed with free-ranging bottlenose dolphin plasma collected in September 2017, the second is an endogenous precision experiment using pooled samples collected in 2012 from dolphins maintained by the United States Navy, and the final experiment is a matrix (serum/plasma) comparison utilizing samples collected from free-ranging dolphins between 2014 and 2016. This dataset supports the publication: Galligan, T. M., Schwacke, L. H., Houser, D. S., Wells, R. S., Rowles, T., & Boggs, A. S. P. (2018). Characterization of circulating steroid hormone profiles in the bottlenose dolphin (Tursiops truncatus) by liquid chromatography–tandem mass spectrometry (LC–MS/MS). General and Comparative Endocrinology, 263: 80–91. doi:10.1016/j.ygcen.2018.04.003
本数据集包含普通宽吻海豚血浆的液相色谱-串联质谱（liquid chromatography tandem mass spectrometry，LC-MS/MS）数据。该类数据采集于2017年1月至12月，旨在评估反相固相萃取（reverse phase solid phase extraction，SPE）耦合液相色谱-串联质谱（LC-MS/MS），以同时、精准（相对标准偏差<15%）且准确（加标回收率为70%~120%）地测定海豚血浆中类固醇激素的应用效果。本数据集涵盖基于三组不同样本集开展的三项独立实验的数据：第一项为加标回收实验，采用2017年9月采集的野生宽吻海豚血浆完成；第二项为内源性精准度实验，使用2012年采集自美国海军饲养海豚的混合样本；第三项为基质（血清/血浆）比对实验，利用2014年至2016年采集的野生海豚样本。本数据集支撑以下发表文献：Galligan, T. M.、Schwacke, L. H.、Houser, D. S.、Wells, R. S.、Rowles, T. 及 Boggs, A. S. P.（2018）。《液相色谱-串联质谱（LC-MS/MS）解析宽吻海豚（Tursiops truncatus）循环类固醇激素谱特征》，《普通与比较内分泌学》（General and Comparative Endocrinology），263卷：80–91。doi:10.1016/j.ygcen.2018.04.003
