Impact of Palbociclib (PD-0332991; CDK4/6 inhibitor) on gene expression of MCF7 cells

Studying DNA damage-induced senescence, we observed a similar phenotype upon CDK4/6 inhibition in breast cancer cells. We used RNA sequencing to investigate the gene expression profile of MCF7 cells upon CDK4/6 inhibition five days after exposure. MCF7 cells were exposed or unexposed for 120 h to 1 µM Palbociclib (PD-0332991, inhibiting CDK4/6). We then performed comparative gene expression profiling analysis using RNA-seq data obtained from 3 independent experiments of exposed and unexposed cells.