ISEVxTECH FCS and PDF data

Lyophilised GFP exosome reference standard provided by ISEVxTech from Merck (Exosome standards, fluorescent recombinant, expressed in human cells | Sigma-Aldrich (sigmaaldrich.com)). <br> Unless otherwise mentioned all samples and standards had data acquired over one minute and where water is used as a diluent it is HPLC grade water. Lyophilized Merck rEV’s resuspended in 100µl of water then placed on ice (all subsequent sample handling performed on ice as per manufacturer instruction). 2.17E+10 particles per ml (P/ml) QC beads diluted 1 in 100 with water to a volume of 100µl. Used to align Nanoanalyzer and create the concentration standard used to assess the samples. Size bead cocktail (68nm, 91nm, 113nm and 155nm) of silica nanospheres diluted 1 in 100 to a volume of 100µl with water then analysed to create the size standard (figure 1). 100µl TE buffer was analysed in order to provide a blank for the samples. Samples were serially diluted 1 in 25, 1 in 50 and 1 in 100 dilutions with TE buffer then immediately analysed in triplicate. Data analysis performed using the nFCM professional software, files saved in FCS. format with associated PDF reports found on Figshare.