Differentially expressed genes in SCC13 NRF3-KO cells and SCC13 cells that re-express endogenous concentrations of recombinant NRF3

We identified a tumor-suppressing function of NRF3 in skin cancer. To determine the underlying mechanism of action we wanted to compare the transcriptome of SCC13 NRF3-KO cells and SCC13 cells that re-express endogenous concentrations of recombinant NRF3. Overall design: First Crispr/Cas9 KO of NRF3 was performed in SCC13 cells whereafter the cells were transduced with a pInducer20 contrusct containing the NRF3 cDNA, which allows doxycycline-inducible re-expression of NRF3. The cells were treated with DMSO (vehicle) or 20 ng/ml Dox to allow for endogenous expression of NRF3 and translocation to the nucleus. Differentially expressed genes were identified using RNA-seq.