Raw data for genome assembly of Myrica nana

Fresh young leaves used for genome sequencing were collected from the M. nana plant grown in the wild environment of Guizhou, China. The total genomic DNA for each of the accessions was extracted from leaves using the CTAB method[20]. A second-generation sequencing library was constructed using the DNBSEQ-T7 platform (MGI, Shenzhen, China), yielding 17.97 Gb of raw data. High-quality genomic DNA was used to construct a SMRTbell library with insert sizes of 15-20 kb, which was sequenced on the PacBio Sequel II platform, generating 16.15 Gb of long-read data. To assist in genome scaffolding and chromatin interaction analysis, a Hi-C library was prepared and sequenced on the same DNBSEQ-T7 platform, resulting in 25.63 Gb of valid data after quality control. In addition, total RNA was extracted from four tissues (root, stem, leaf, flower, and fruit) using TRIzol reagent (Vazyme, China). A cDNA library with an insert size of 350 bp was constructed using the MGI Easy RNA Library Prep Kit and subjected to paired-end sequencing on the DNBSEQ-T7 platform, producing 37.77 Gb of transcriptomic raw data.