Immunostaining images from CRC patients stained with AGR2 and SGTA antibodies

The Endoplasmic Reticulum (ER) is an essential sensing organelle responsible for the folding and secretion of almost one-third of eukaryotic cells' total proteins. However, environmental, chemical, and genetic insults often lead to protein misfolding in the ER, accumulating misfolded proteins, and causing ER stress. To solve this, several mechanisms were reported to relieve ER stress by decreasing the ER protein load. Recently, we reported a novel ER surveillance mechanism by which proteins from the secretory pathway are refluxed to the cytosol to relieve the ER of its content.  The refluxed proteins gain new prosurvival functions in cancer cells, thereby increasing cancer cell fitness. We termed this phenomenon ER to CYtosol Signaling (or \"ERCYS\"). Here, we found that in mammalian cells, ERCYS is regulated by DNAJB12, DNAJB14, and the HSC70 cochaperone SGTA. Mechanistically, DNAJB12 and DNAJB14 bind HSC70 and SGTA - through their cytosolically localiz..., , , # Immunostaining images from CRC patients stained with AGR2 and SGTA antibodies

[https://doi.org/10.5061/dryad.h70rxwdvs](https://doi.org/10.5061/dryad.h70rxwdvs)

## Description of the data and file structure

This dataset is presented in Figure S5F-G as analysed images, here we provide the raw images as well in triplicates. The aim of these images is to provide an evidence that the cytosolic HSC70 co-chaperone colocalizes with  othe ER proteins AGR2 in tumor isolated from Colorectal cancer patients. Those human samples used for the analyses shown in here were provided by the the Colorectal cancer (CRC) biobank in Soroka, Beer Sheva. the samples were collected after Helsinki approval of biobank samples collection, approval number 0137-18. The samples were fixed with 4% paraformaldehyde in PBS for 20 minutes at room temprature. After three washes, samples were permeabilized with  0.5% triton x-100 for 15 min and then washed twice with PBS. 10% goat serum was added for 1hour at room te...,