Expression data from hiPS-derived lung organoids

Lung organoids made from pluripotent stem cells have the potential to enhance our understanding of disease mechanisms in pediatric lung disorders. As proof of concept, we have established a reproducible ex vivo model of lung organoid development derived from human induced pluripotent stem cells generated from fetuses and infants with Bockdalek congenital diaphragmatic hernia (CDH), a polygenic disorder associated with fetal lung compression and often lethal pulmonary hypoplasia at birth. We used microarrays to compare transcriptomes among the different cell types focusing on genes associated with lung development and extracellular matrix. We seek to identity anomalous gene expression during lung development, using lung organoids generated from hiPS of patients with congenital diafragmatic hernia. RNA from dermal fibroblasts, iPS cells, and lung organoids (20, 40 and 60 days in vitro) from normal controls and CDH patients was obtained and hybridized on Affymetrix microarray. Two replicates of each sample (control and CDH) were analyzed and compared to one human fetal lung sample (total 20 samples).