Potential modulation of Puf3 chromatin association mediated by Rpb4. Potential modulation of Puf3 chromatin association mediated by Rpb4

Gene expression involving RNA polymerase II is regulated by the concerted interplay between mRNA synthesis and degradation, a crosstalk where mRNA decay machinery impacts transcription and transcription machinery influences mRNA stability. Rpb4, and likely the dimer Rpb4/7 seem to be the central components of the RNA pol II governing these processes. Here we investigate the effect of ∆rrp4 on the potential chromatin association of the RNA binding protein Puf3 to chromatin in S. cerevisiae. Overall design: ChIP-seq was performed for 8 samples: 3 biological replicates for PUF3-TAP WT and PUF3-TAP Δrpb4 strains, and 2 biological replicates for the WT strain. For each sample, input was also sequenced.