Identification and characterization analysis of the different genes in primary neurons response to circSCMH1 using RNA-seq.. Identification and characterization analysis of the different genes in primary neurons response to circSCMH1 using RNA-seq.

Whole RNA-sequencing analysis was performed by LC-Bio. Day-10 neurons from the Vector or circSCMH1 overexpression groups were collected in Trizol. Total RNAs were extracted from Trizol. UMI technology was used to label each sequence fragment with sequence tags, which minimizes the interference of duplication generated by PCR amplification on the quantitative accuracy of transcriptome. RNA-seq reads were aligned to the mouse genome (GRCh37/hg19) using the software Hisat2 (2.0.4). Transcript abundance was quantified as fragments per kilo base of exon per million fragments mapped (FPKM). Differentially expressed genes were determined by DESeq2 (DOI: 10.1186/s13059-014-0550-8). Overall design: Examination of different genens in circSCMH1-treated primary neurons by RNA-seq.