Heliopsis helianthoides seed de novo transcriptome

PawS1 (Preproalbumin with SFTI-1) is subjected to an unusual biosynthesis whereby a small trypsin-inhibitory cyclide peptide, SFTI-1 (Sunflower Trypsin Inhibitor-1) is processed from it in addition to a napin-type 2S albumin protein in sunflower seeds. Two PawS genes, PawS1 and PawS2 were identified in sunflower. De novo transcriptomics of seed RNA identified a PawS-Like gene (PawL1) in sunflower and Arnica montana (Elliott et al., 2014, Plant Cell 26: 981-995). The proteins encoded by PawS-Like genes share many features of PawS1, but PawL1 is matured only into a napin and no stable peptide is formed. By using a de novo transcriptomics approach we identified the three PawS1 genes in Heliopsis helianthoides seeds which were previously described by Elliott et al., 2014, Plant Cell 26: 981-995 and a novel PawL1 transcript.