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RNA-seq profiling was performed on murine primary lung fibroblasts and their TGF-beta-induced myofibroblast derivatives.

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NIAID Data Ecosystem2026-05-10 收录
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Primary lung fibroblasts were isolated from C57BL/6 mice by enzymatic digestion and confirmed by immunofluorescence staining to be >=95 % pure. Cells were then exposed to 10 ng/mL TGF-beta for 12 hours to generate a fibrotic model. RNA-seq was subsequently used to analyze the transcriptomic response of these fibroblasts to TGF-beta stimulation.

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2026-03-01
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