Gene expression changes after Ctcfl overexpression in undifferentiated and differentiated p19 cells.

The two paralogous zinc finger factors CTCF and CTCFL differ in expression such that CTCF is ubiquitously expressed, whereas CTCFL is found during spermatogenesis and in some cancer types. Both factors share the highly conserved DNA binding domain and are bound to DNA sequences with an identical consensus. Here we analyzed the differential gene expression effect mediated by expression of Ctcfl in undifferentiated and differentiated p19 cell. p19 wt cells and p19 cells stably transfected with inducible Ctcfl expression vectors were used for RNA extraction and hybridization on Affymetrix microarrays. We analyzed gene expression in the presence/absence of Ctcfl in undifferentiated as well as as cells differentiated with 10 µM retinoic acid.