小鼠创伤性脑损伤后皮质细胞的单细胞测序

Traumatic brain injury (TBI) can lead to significant neuropsychiatric problemsand neurodegenerative pathologies, which develop and persist years after injury. Neuroinflammatory processes evolve over this same period. Cortical mRNA analysis showed a robust contribution of microglia to neuroinflammatory pathways that persisted over time post-injury. These data also indicate that inflammation persists in the subacute and chronic time points after TBI, which may affect surrounding neurons, oligodendrocytes and astrocytes. To investigate this hypothesis, a single-cell sequencing approach was used to determine cell-type specific gene expression within the cortex 7 dpi with and without microglial depletion.

创伤性脑损伤（traumatic brain injury, TBI）可引发严重的神经精神障碍与神经退行性病变，此类病变会在损伤发生数年后出现并持续存在。神经炎症过程会在这一病程周期中同步演变。皮层mRNA分析显示，小胶质细胞在损伤后随时间持续存在的神经炎症通路中发挥了关键作用。上述数据同时表明，创伤性脑损伤后亚急性与慢性阶段的炎症反应仍持续存在，该炎症可能会对周围的神经元、少突胶质细胞与星形胶质细胞造成影响。为验证该假说，本研究采用单细胞测序技术，分别检测了存在与不存在小胶质细胞耗竭的情况下，损伤后7天（days post injury, dpi）的皮层组织内细胞类型特异性基因表达情况。

搜集汇总

背景与挑战

背景概述

该数据集通过单细胞测序研究小鼠创伤性脑损伤后皮质细胞的基因表达变化，重点关注小胶质细胞介导的神经炎症过程。包含8个8-10周龄小鼠样本的测序数据，使用Illumina HiSeq 2000平台生成，旨在揭示TBI后亚急性和慢性期的持续炎症对神经细胞的影响。