Multi-element amendment reshapes rhizosphere microbiome: A microbially-driven Fe/Mn/S synergistic action for Cd immobilization

Background: Cadmium (Cd) contamination in agricultural soils poses a significant threat to rice safety and global food security. While multi-element amendments are a promising strategy to reduce Cd bioavailability, the underlying microbial mechanisms remain largely underexplored. This project investigates how a silicon-calcium-magnesium based multi-element amendment (FSY) reshapes the rice rhizosphere microbiome to drive Cd immobilization.Study Design: The study was conducted using Cd-contaminated paddy soil in rhizotrons. We established two treatment groups: a control (CK) and an FSY-amended group (0.3% w/w), with six replicates each. Rhizosphere soil samples were collected from three distinct depth layers (0-10 cm, 10-20 cm, 20-30 cm) at two key rice growth stages: the jointing stage and the maturing stage. This design allows for the investigation of spatiotemporal dynamics of the microbial community in response to the amendment.Sequencing and Data Type: Total DNA was extracted from the rhizosphere soil samples. Shotgun metagenomic sequencing was performed on the Illumina NovaSeq 6000 platform (PE150). This dataset consists of raw sequence reads from the rhizosphere microbial communities, which can be used to analyze taxonomic composition, functional gene potential, and microbial interaction networks.Overall Goal: The primary goal of this project is to elucidate the key microbial taxa (including low-abundance species), functional genes, and synergistic biogeochemical pathways (Fe/Mn/S-Cd interactions) that contribute to enhanced Cd immobilization under FSY treatment. This dataset provides a valuable resource for researchers studying soil remediation, microbial ecology, biogeochemistry, and sustainable agriculture.