Triplication of the Ifnr locus exacerbates craniofacial anomalies in a mouse model of Down syndrome.

Micro-computed tomography (mCT) reconstructions of skulls from the Dp16 mouse model of Down syndrome, Dp16 corrected for Ifnr dosage (Dp162xIfnrs), and wildtype controls (WT). Mice were decapitated at 7-8 weeks of age then whole heads imaged using a SkyScan 1275. Scanning was performed at 17.6-micron resolution using the following parameters: 55 kV, 180 mA, 0.5 mm Al filter; 0.3˚ rotation step over 180˚, and 3-frame averaging. All raw scan data were reconstructed to multiplanar slice data using NRecon v1.7.4.6 (Bruker, Belgium). Reconstructed data were then rendered in 3D with consistent thresholding parameters using Drishti v2.6.5 for gross visual assessment of the craniofacial skeleton. Representative rendered images were captured and processed using Photoshop 24.2.0 (Adobe Creative Cloud).