Efficient arming of memory-like NK cells with a TCR-like CAR for treating acute myeloid leukemia with NPM1 mutation

We showed Cytokine induced memory-like (CIML) human NK cells have higher trasduction efficiency with CAR lentivirus for therapeutics. We performed single cell RNAseq analysis to comprehensively understand the transcripttion difference between NPM1c-CAR-mb15 CIML NK cells and CAR-negative CIML NK cells, as well as their transcriptional response to NPM1c+ AML tumor cells. CIML NK cells were generated from purified NK cells from human PBMC (two different donors) in vitro and then transduced with NPM1c-CAR-mb15 lentivirus. Single cells (mixture of transduced and non-transduced) post 5 days transduction were immmediately applied to single cell RNAseq. At the same time, CIML NK cells were cocultured with OCI-AML3 target tumor cells for 24 hrs and then applied to single cell RNAseq.