Dataset for: Functional imaging of nine distinct neuronal populations under a miniscope in freely behaving animals

This record contains raw and processed data supporting:"Functional imaging of nine distinct neuronal populations under a miniscope in freely behaving animals" Imaging modalities:- Miniscope calcium imaging (nVoke2)- In vivo multiplexed confocal spectral imaging (LSM 980) Folder descriptions: 4FluorophoreData/: Reduced-complexity experiments using four retrograde fluorophores, organized by animal ID. Inscopix/: CNMF-E spatial maps and anatomical reference images, organized by imaging day ZEISS/: Confocal spectral images and imported ROIs 9FluorophoreData/: Full Neuroplex experiments using nine injected fluorophores (plus GCaMP), organized by animal ID. Behavior/: Timestamped behavioral probabilities for each session Inscopix/: CNMF-E spatial maps and anatomical reference images, organized by imaging day ZEISS/: Confocal spectral images and imported ROIs ProcessedData/: ROI definitions, raw and normalized spectral fingerprints, and fluorophore assignments CalibrationData/: Optical calibration and validation datasets. ArgoSlide: Fluorescent calibration slide data used for image-to-image registration and GRIN lens aberration characterization Hek293Cells: In vitro spectral imaging data from HEK293T cells expressing individual fluorophores, used to generate pure fluorophore spectral fingerprints Corresponding analysis code available at: GitHub - Neurocipher/PythonPipeline: Python pipeline for Neurocipher