Gene expression analysis of alveolar macrophages tolerized to ozone

In this study, we examined the effect of repeated exposure to the air
pollutant ozone (O3) on the transcriptome of alveolar macrophages (AMs).
Using flow cytometry, we isolated purified AMs from the lungs of female
C57BL6/NJ mice exposed to filtered air or 0.8 ppm O3 for four days and
then collected 2 days later, with six mice per group. RNA was isolated
from AMs and then subjected to RNA-seq analysis. We performed differential
gene expression analysis followed by gene ontology enrichment analysis of
differentially expressed genes.