AP-MS of MAGU-2 in the nematode C. elegans

The Crumbs complex is a regulator of epithelial polarity and is important for the formation of the apical domain. The Crumbs complex consists of the Crumbs protein, Pals1, and PATJ. In C. elegans, we identify MAGU-2 as an ortholog of Pals1. We find that MAGU-2 localizes apically in epithelial cells and its localization is dependent on Crumbs. AP-MS was performed on animals endogenously expressing MAGU-2::GFP and four control strains to identify interactors of MAGU-2. From the data, we identify MPZ-1 as the top hit and identify it as the C. elegans ortholog of PATJ.