Transcriptome profiles of colonic stem cells of WT and AHR deficient mice treated with either DMSO or high affinity AHR ligand, FICZ for 4 hours

To identify aryl hydrocarbon receptor (AHR) dependent transcriptional changes in mouse colon stem cells, we performed RNA sequencing of colon organoids from wildtype and AhR deficient mice stimulated with an high affinity AHR ligand, 5nM FICZ for 4 hours. Overall design: Organoids were prepared from colonic crypts of WT and AHR deficient mice and maintained in stem cell condition with WENR medium. Fully grown organoids were treated with either DMSO or 5nM FICZ for 4 hours in vitro and RNAseq was performed on total RNA.