57Fe Wall Loss Experiment data collected as part of a method development study investigating the precipitation and wall loss of labeled 57Fe when added to M9 Minimal Media

<p>This data was collected as part of a method development study investigating the precipitation and wall loss of labeled ⁵⁷Fe when added to M9 Minimal Media, which is used to grow the model bacterial species <em>Escherichia coli.</em> The bulk media was prepared with the same components and a portion was treated with Chelex-100 to remove metals, while the other portion remained un-chelexed. Half of each treatment was spiked with labeled ⁵⁷Fe and either 0.2 μm or 0.02 μm filtered for comparison of the dissolved and soluble fractions. The ⁵⁷Fe content was monitored over four time points for one week in a shaking incubator, under the same conditions used to culture <em>E. coli</em> for labeling experiments.</p>本数据集源自一项方法学开发研究，该研究针对标记性铁-57（⁵⁷Fe）添加至用于培养模式细菌大肠杆菌（Escherichia coli）的M9最小培养基（M9 Minimal Media）后的沉淀行为与器壁损耗情况展开探究。基础培养基采用统一组分制备，其中一份经Chelex-100（Chelex-100）处理以去除金属杂质，另一份则未进行螯合脱金属操作。每组处理的半数样本添加标记性铁-57后，分别以0.2 μm或0.02 μm孔径滤膜过滤，用于对比溶解态与可溶性组分的差异。在与大肠杆菌标记实验所用培养条件完全一致的振荡培养箱中，于一周内的四个时间点对体系内铁-57的含量进行监测。