Comprehensive analysis of aberrantly expressed circRNAs, mRNAs and lncRNAs in patients with nasopharyngeal carcinoma

The location of nasophryngeal cancer is hidden,so ti is difficult to diagnose at an early stage.In this study,we aimed to investigate expression profiles of circRNAs,mRNAs and IncRNAs and to provide some basis for further study.The expression profiles of circRNAs, mRNAs and lncRNAs were analyzed by microarray technology. The differentially expressed ncRNA was calculated by bioinformatics. Our study characterized the landscape of circRNAs, mRNAs and lncRNAs in NPC tissue and provided novel insights into the molecular mechanisms of NPC. We collected fresh tissue samples of NPC (n =4) and the chronic inflammation of the nasopharyngeal mucosa tissues (n =4).Total RNA was extracted from the frozen tissue specimens using TRIzol reagent (Invitrogen, Grand Island, NY, USA) according to the manufacturer’s instructions, and purified by using a RNeasy Mini Kit (Qiagen, GmBH, Germany).Total RNA was checked for a RNA integrity number (RIN) to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). The quality and amount of RNA were assessed using a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Rockford, IL, USA).