Bulk and single cell RNA sequencing analyses of GBM Primary and Recurrent, early and late passages, DMSO / TMZ treatment.

We used bulk RNA sequencing to analyze unsupervised clustering of early and late passages of four GBM PDXs. Single cell RNAseq data was used to study transcriptional profiles of DMSO and TMZ, Primary and Recurrent as well as early and late passages of GBM PDXs. Patient PDX cells treated with DMSO or Temozolomide for 5days and subsequently harvested (see STAR Methods section, Xiex et al., 2024).