Isolated from the middle-ear of a child with chronic otitis media. Haemophilus influenzae PittII

Haemophilus influenzae PittII. Strain PittII is a non-typeable Haemophilus influenzae (NTHi) isolate recovered by Dr. Robert Wadowsky from the middle-ear effusion of a child in Pittsburgh who was undergoing tympanostomy and ventilation tube placement for the treatment of chronic otitis media with effusion (COME). In the chinchilla model, PittII infection induces acute otitis media (AOM) and causes virulent, often fatal, systemic disease. Sequenced and assembled using technology developed by 454 Life Sciences, Inc. (www.454.com), supplemented with double-barreled Sanger dideoxy sequencing read data from traditional clone libraries. The genome sequence is currently comprised of 205 contigs, and has been sequenced to a depth of 35X.