The identification of genes altered by RORa in progression of non-alcoholic steatohepatitis (NASH)

Purpose: To identify the target genes of RORa associated with development of NASH Methods: RNA isolation for sequencing analysis from liver tissues of High fat diet (HFD)-fed RORa-LKO and RORa f/f mice and mRNA-sequencing Conclusion: When calcuated by dividing the normalized read count of HFD-fed RORa-LKO by that of RORaf/f,.there are 8505 genes altered by RORa deletion (cut-off, 0.8-fold, 1.5-fold). Overall design: mRNA profiles of HFD-fed control (RORa f/f) and RORa liver-specific KO (LKO) mice