Transcriptome analysis using RNA sequencing conducted for wild type (Col-0), ahl10-1 and rrp6l1-2 mutant under control conditions and after 96 h at moderate low water potential (-0.7 MPa)

RNA sequencing was used to identify genes differentially expressed (DEG) in ahl10-1 mutants and rrp6l1-2 mutant compared to Col-0 wild type. Two treatments were analyzed for each genotype: unstressed control and a 96 hour low water potential (-0.7 MPa) treatment. Approximately 2000-3000 genes were found to be significantly up and down-regulated in the wild type stress versus control treatment using . rrp6l1-2 mutant shows around 500 and 250 genes up and down regulated respectively in control treated seedlings while 10 or less than 10 found in ahl10-1. In the stress treatment, rrp6l1-2 and ahl10-1 have less and 60 genes up and downregulated. ahl10-1 and rrp6l-2 have a common set of downregulated genes (24 genes) at stress treatment. Wild type (Col-0), ahl10-1 and rrp6l1-2 Arabidopsis thaliana seedlings were germinated on agar plates and after seven days of growth, seedlings were transferred either to fresh plates of control media or to moderate severity low water potential (-0.7 MPa) imposed using agar plates of the control media infiltrated with PEG-8000. Samples were collected 96 h after transfer (Control and stress treatments). Three independent biological experiments and mRNA extractions were performed for each genotype/treatment. (18 mRNA extractions. After quality check and quantitation the 18 samples were used for sequencing