HYP1-dependent changes in the root transcriptome of Arabidopsis thaliana in response to phosphorus deficiency

Phosphorus (P) is essential for undisturbed plant growth and development. However, in most soils, total amounts of P are very low or the P that is present is poorly available to plants. We identified HYPERSENSITIVE TO LOW P1 (HYP1; AT5G35735) as critical to maintain cell elongation and meristem integrity in roots of Arabidopsis thaliana grown under low P. HYP1 is a CYBDOM protein sharing structural similarities with ferric reductases of animal cells. To investigate which genes and gene regulatory networks are dependent on HYP1 activity, we assessed genome-wide transcriptional responses to low P in roots of wild-type plants (Col-0), in a T-DNA insertion mutant disrupted in HYP1 expression (hyp1), and in a transgenic line overexpressing HYP1 (HYP1-OX). Total RNA was extracted with a kit (NucleoSpin® RNA II; MACHEREY-NAGEL) following manufacturer's instructions. Library construction and sequencing were done at Novogene (UK) Company Limited. Briefly, RNA integrity and quantitation were determined with the Agilent 5400 Bioanalyzer. Total mRNA was enriched with oligo(dT) beads and randomly fragmented with fragmentation buffer. A stranded specific library was then prepared for each sample and sequenced with a NovaSeq 6000 PE150 platform.