Conservative aa substitutions occurring during long-term replication of genome-length HCV RNAs (I).

aConservative aa substitutions detected in at least two of four cell line series.
bConservative aa substitutions detected in HuH-7-derived cell line series (O, OA, OB, OD, or OE) used in the previous study [20].
cConservative aa substitutions that became the same aa as the JFH-1 strain.
Conservative aa substitutions detected after 2-year and 4-year cultures are shown by bold letters.
Conservative aa substitutions detected only after 2-year culture are shown within parentheses.