mRNA expression analysis of irradiated skin tissues of mice

Radioactive materials have been widely used in industry, medicine, science, the military and nuclear facilities, which has significantly increased the potential of large-scale, uncontrolled exposure to radiation. The skin is one of the radiosensitive organ systems and radiation-induced skin injury remains a serious concern after ionizing radiation exposure. This study aims to illustrate the early response (mRNA profiles) of skin tissues following radiation and find targets against radiation-induced skin injury. Overall design: Male C57 mice (4 weeks old) were irradiated with a single 35-Gy dose at a rate of 750 cGy/min using a 6-MeV electron beam accelerator (Clinac 2100EX, Varian Medical Systems, Inc., CA). After 24 h, skin tissues were collected. Skin tissues from nonirradated skin areas and irradiated areas were collected and subjected to RNA sequencing.