A Porcine Model of Heart Failure With Preserved Ejection Fraction Induced by Chronic Pressure Overload Characterized by Cardiac Fibrosis and Remodeling

In this study, we employed descending aortic constriction (DAC) technique to establish a minipig heart failure model. The cardiac phenotypes, pathological and cellular changes, fibrotic signaling activation, and gene expression profiles have been explored. Overall design: Total RNAs from the ventricular tissues of Sham (n=3) and DAC (n=3) minipigs were isolated using TRIzol® Reagent. Each group contains three independent samples. Subsequently, a total amount of 3 µg RNA per sample were used for transcriptome sequencing.