Significance testing results for 16 S rRNA and MLST markers in a five organism model.

1sKH test results across the 15 candidate tree topologies presented in Figure 4B with respect to different marker sequence alignments. For the protein-encoding marker genes gidA and sucB both the hypervariability filtered nucleotide and the deduced amino acid sequence levels were explored. For the 16 S rRNA marker, two data sets comprising sequences from different Rickettsiella grylli strains were analyzed. The p-values attributed to a tree topology by the 1sKH test are interpreted under assumption of an exogenous 5% significance threshold as “B” for a data set specific best tree, “U” for an unrejected second-best tree, and “R” for a rejected tree. Data set specific best trees are indicated in bold type.