Additional file 2: of Biofilm production by Haemophilus influenzae and Streptococcus pneumoniae isolated from the nasopharynx of children with acute otitis media

Supplementary Data S2: Different cut-offs used to determine the biofilm production by H. influenzae: the different cut-offs used were as followed where AB represented the stained wells containing attached bacteria, CW the stained control wells containing bacteria-free medium only and G the bacterial growth control i) AB – CW: strong production > 0.30, moderate production 0.10–0.30, negative < 0.10 ii) AB / CW: strong production > 6, moderate production 2–6, negative < 2 iii)[(AB – CW) / G]: strong production > 1.10, moderate production 0.35–1.10, negative < 0.35. Strains were classified according to agreement of at least 2 of the 3 methods used to calculate biofilm production. (XLSX 76 kb)