Data from: Early molecular oxidative stress biomarkers of ischemic penumbra in acute stroke

Objectives: To assess whether plasma biomarkers of oxidative stress
predict diffusion-perfusion mismatch in acute ischemic stroke (AIS)
patients. Methods: We measured plasma levels of oxidative stress
biomarkers such as F2-isoprostanes (F2-isoP), total and perchloric acid
Oxygen Absorbance Capacity (ORACTOT and ORACPCA), urinary levels of
8-oxo-7,8-dihydro-2’-deoxyguoanosine, and inflammatory and tissue-damage
biomarkers (high-sensitivity CRP, matrix metalloproteinase 2 and 9), in a
prospective study of AIS patients presenting within 9 hours of symptom
onset. Diffusion- (DWI) and perfusion-weighted (PWI) MRI sequences were
analyzed using a semi-automated volumetric method. Mismatch was defined as
baseline mean transit time volume minus DWI volume. A percent mismatch
cut-off of >20% was considered clinically significant. A stricter
definition of mismatch was also used. Mismatch salvage was the region free
of overlap by final infarction. Results: Mismatch >20% was present
in 153/216 (70.8%) patients (69.2±14.3 years; 41.2% women). Patients with
mismatch >20% were more likely to have higher baseline plasma
levels of ORACPCA (P=0.020) and F2-isoP (P=0.145). Multivariate binary
logistic regression demonstrated that lnF2-isoP (OR 2.44 95% CI 1.19-4.98;
P=0.014) and lnORACPCA (OR 4.18, 95% CI 1.41-12.41; P=0.010) were
independent predictors of >20% PWI-DWI mismatch and the stricter
mismatch definition, respectively. lnORACTOT, significantly predicted
mismatch salvage volume (>20% mismatch: P=0.010; stricter mismatch
definition: P=0.003). Conclusions: Elevated hyperacute plasma levels of
F2-isoP and ORAC are associated with radiographic evidence of mismatch and
mismatch salvage in AIS subjects. If validated, these findings may add to
our understanding of the role of oxidative stress in cerebral tissue fate
during acute ischemia.