Single-cell transcriptomic profiling of brain injury induced by extracorporeal life support

To investigate the cellular and molecular mechanisms underlying brain injury during extracorporeal life support (ECLS), brain tissue samples were collected following ECLS exposure and subjected to single-cell RNA sequencing using the 10X Genomics platform. This dataset provides a high-resolution transcriptional landscape of brain-resident cell populations, including neurons, glial cells, and immune cells, under ECLS conditions.Single-cell transcriptomic analysis was performed to characterize cell-type-specific transcriptional changes, cellular heterogeneity, and alterations in inflammatory and stress-related pathways associated with ECLS-induced brain injury. The resulting dataset enables systematic exploration of cellular responses and intercellular communication networks in the brain following ECLS, serving as a valuable resource for understanding the pathophysiology of ECLS-related neurological complications and for identifying potential therapeutic targets.