Transcriptional analysis of cystic fibrosis airways at single-cell resolution reveals altered epithelial cell states and composition

Single cell sequencing of human airway epithelium from normal and cystic fibrosis lungs. Single cell RNA-seq using dropSeq (UCLA) or 10x chromium system (CFF, CSMC), on freshly isolated (UCLA, CFF), FACS isolated (DAPI-,CD45-,CD31-,CD326+) (CSMC), or freshly isolated, expanded, and cultured on ALI for 28 days (CFF) epithelial cells from control and CF lung explant tissue. The barcoded sequencing libraries were quantified by qPCR using the KAPA Library Quantification Kit (KAPA Biosystems). Sequencing libraries were loaded on a NovaSeq 6000 (Illumina) (CSMC), NextSeq 500 (Illumina) (CFF) or HiSeq4000 (Illumina) (UCLA).