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Large-scale purification of HTT domain constructs 2019/04/01

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NIAID Data Ecosystem2026-05-02 收录
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https://zenodo.org/record/2628059
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Project: High resolution structural analysis of purified HTT samples Experiment: Large-scale purification of HTT domain constructs Date completed:­ 2019/04/01 Rationale: Domain fragments of the huntingtin protein are useful reagents for examining protein-protein interaction characteristics of huntingtin and to map interaction interfaces. Stable, monodisperse and pure samples may also be amenable to high resolution structure solution by X-ray crystallography. Previously huntingtin fragments were cloned and screened for expression in small-scale (3 mL) culture experiments. Positive hits were scaled for production as detailed in the table on the next page.   All previous work is described in this post:https://zenodo.org/record/2600051#.XKU89aeZPOQ.
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2024-07-24
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