Effect of miR-33 supression in hepatocytes in NAFLD and NASH

We perform sc-RNAseq in NASH livers from WT and miR-33-AlbCRE conditional knock-out mice. Liver were isolated from mice after 6 months of feeding a choline deficient high fat diet to analyze the effect of miR-33 deficiency specifically in hepatocytes and the crosstalk between hepatocytes and other cell types in the liver. Liver single cell suspension from WT and mir-33 HKO mice after 6 months of CD-HFD feeding to indunce NASH.