Oncogenic KRAS-dependent stromal interleukin-33 directs the pancreatic microenvironment to promote tumor growth [bulk RNA-seq]

This experiment was designed to investigate the intrinsic effect of interleukin-33 (IL-33) on pancreatic cancer associated fibroblasts. IL-33 wildtype (WT) or IL-33 knockout (KO) fibroblasts were grown with or without tumor conditioned media to stimulate cancer-associated fibroblast (CAF) polarization and harvested for bulk RNA sequencing. Overall design: IL-33 WT or IL-33 KO fibroblasts were treated for 24h in 1% FBS DMEM or a 1:1 ratio of 1% FBS DMEM + 1% FBS 9805 tumor cell conditioned media. Doxycycline was provided to all groups as a control for 9805 culture conditions. RNA was harvested and submitted for bulk RNA sequencing.