High throughput phenotypic screen and transcriptional analysis identify new compounds, targets and pathways for macrophage reprogramming III
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https://www.ncbi.nlm.nih.gov/sra/SRP247847
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Purpose: To understand the effects of signaling ligands on human macrophage polarization in the transcriptional level Overall design: Fresh isolated human blood monocytes were cultured with 50ng/mL M-CSF for 7 days to generate M0 status macrohages. Macrophages were treated with serontonin, dopamine, vegfa, egf and leptin for 24 hrs. RNA was isolated and purified by Qiagen Rneasy micro kit and applied to RNAseq.
创建时间：
2021-02-17
