Antifungal Benzimidazole Derivative Response

Benzimidazol-2-yl-alkylamine derivative showed the highest antifungal activity and best compatibility with human cells against a number of different yeasts and clinical isolates. The statistical analysis of the data revealed significant differences between the treated C. albicans SC5314 culture and the untreated reference culture. Several genes had changed transcript levels in response to antifungal derivatives.

3 biological replicates were performed in total. All experiments were performed as dye swaps, for a total of 6 hybridized arrays. Hybridization experiments included an untreated reference sample and a sample of cells treated with ((1S)-1-[1-(3-chlorobenzyl)-1H-benzimidazol-2-yl]-2-methylpropyl-amine) (EMC120B12). The array included one technical replicate of each probe.