Effect of chronic social defeat stress (CSDS) on gene expression in wild-type mice and in Neurensin-2 knockout mice.

CSDS is a robust model of depression with a detective behavioral effect. In prior studies, we established that the deletion of Neurensin-2, a vesicular protein, confers resilience in this model. The distinctive behavioral effect of CSDS stems from underlying molecular stress-induced changes, including changes in gene expression. To understand the mechanism in which deletion of Neurensin-2 confers resilience, we analysed the stress-induced changes in gene expression within each genotype. Overall design: To investigate the stress-induced gene expression changes, we harvested hippocampi from wild-type and Neurensin2 -knockout mice, at base line and after CSDS. 5 for control and 6-7 after CSDS On the RNA, which was extracted from the hippocampi lysates, we preformed sequencing, gene expression profiling and pathway analysis.