Sample data for analysis of sequence variation in HIV
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These are downsampled interleaved paired fastq datasets from Jair et. 2019 (https://doi.org/10.1371/journal.pone.0214820). The datasets were prepared by: Downloading original data from NCBI SRA (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA517147) Trimming contaminating Nextera adapters using trim-galore Mapping reads against nxb2 reference of HIV genome (K03455.1) with BWA MEM Restricting mapped reads to <em>pol</em> gene vicinity (K03455.1:2000-5100) Downsampling mapped data to ~10% of the original with Picard's DownsampleSam Converting BAM to Interleaved Fastq with Picard's SamToFastq Gzipping resultant interleaved paired fastq files
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Zenodo创建时间：
2019-10-25
